AOD-9604 reconstitution calculator.
A 2 mg vial of AOD-9604 dissolved in 2 mL of bacteriostatic water is 1,000 mcg per mL, which is 10 mcg per unit on a U-100 insulin syringe.
AOD-9604 was developed as an oral anti-obesity drug and its clinical program was discontinued, so there is no labeled injectable presentation to compare a vial against. It is sold in the same 2 mg and 5 mg vials as HGH fragment 176-191 and often treated as the same compound, which it is not, even though the arithmetic on a matching vial is identical.
Reference range often cited for AOD-9604: 300–500 mcg, shown for orientation only, not a recommendation. Enter your own target below. Read the evidence →
Each unit on a 100u · 1.0 mL syringe ≈ 10 mcg of this solution.
Show the math
More water → lower concentration → more units for the same amount.
How much bacteriostatic water for a AOD-9604 vial?
There is no single correct volume. More water gives a weaker solution, so each unit on the syringe holds less AOD-9604 and a given amount takes more units to measure, which is easier to read on the syringe scale. Less water does the opposite. These tables show the concentration for each common vial size across the usual volumes. They are arithmetic, not a recommendation of how much to take.
| BAC water | Concentration | Per 1 unit | Per 10 units |
|---|---|---|---|
| 1 mL | 2,000 mcg/mL | 20 mcg | 200 mcg |
| 1.5 mL | 1,333 mcg/mL | 13.3 mcg | 133 mcg |
| 2 mL | 1,000 mcg/mL | 10 mcg | 100 mcg |
| 2.5 mL | 800 mcg/mL | 8 mcg | 80 mcg |
| 3 mL | 667 mcg/mL | 6.7 mcg | 67 mcg |
| BAC water | Concentration | Per 1 unit | Per 10 units |
|---|---|---|---|
| 1 mL | 5,000 mcg/mL | 50 mcg | 500 mcg |
| 1.5 mL | 3,333 mcg/mL | 33.3 mcg | 333 mcg |
| 2 mL | 2,500 mcg/mL | 25 mcg | 250 mcg |
| 2.5 mL | 2,000 mcg/mL | 20 mcg | 200 mcg |
| 3 mL | 1,667 mcg/mL | 16.7 mcg | 167 mcg |
How many units of AOD-9604 is a given amount?
Divide the amount in micrograms by the micrograms per unit for your mix. With a solution of 2,500 mcg per mL, one unit on a U-100 syringe holds 25 mcg, so any amount in mcg divided by 25 gives the units. The calculator above does this for you from your own vial and water, and the tables give the per-unit figure for each common mix.
Why does AOD-9604 need its own calculation?
- 5 mg made up to 2 mL is 2,500 mcg/mL, so 250 mcg is 10 units. A 2 mg vial in the same 2 mL is 1,000 mcg/mL, where the same 250 mcg is 25 units.
- The format notes on this page dissolve it in a small volume of dilute acetic acid before adding bacteriostatic water. If you do the same, enter the total volume of both, because that is what sets the concentration.
- AOD-9604 is residues 177 to 191 of growth hormone with a tyrosine added at the front. The site keeps it separate from the 176-191 fragment, and a unit chart from one matches the other only because the vials do.
- It is measured by mass, in mcg. The IU conversion used for somatropin does not apply to it.
How is AOD-9604 administered, and what does the math look like?
How AOD-9604 is actually administered, with the concentration arithmetic written out. Amounts in these tables are illustrative examples of the math, never a recommendation.
AOD-9604: research formats
Choose the format you are researching to see route-specific notes.
GH fragment; requires acetic acid for reconstitution. Similar format to IGF-1 LR3.
AOD-9604 is a modified C-terminal fragment of growth hormone (residues 177 to 191 with an added tyrosine). It is related to, but distinct from, HGH fragment 176–191. Like other basic/aggregation-prone fragments, it requires initial reconstitution in 0.6% acetic acid before diluting with BAC water. Sold as a 2–5 mg vial.
AOD-9604 reconstitution: common questions
How many units is 500 mcg of AOD-9604?
On 5 mg in 2 mL (2,500 mcg/mL) it is 20 units. On a 2 mg vial in 2 mL (1,000 mcg/mL) it is 50 units, half a U-100 syringe.
Is AOD-9604 the same as HGH fragment 176-191?
They are closely related and sold in the same vial sizes, but AOD-9604 carries an added tyrosine, and the site treats their evidence separately. Almost all of the human trial data belongs to AOD-9604, and the two profiles explain the difference.
Was AOD-9604 ever approved?
No. It reached human obesity trials, which did not show meaningful weight loss, and development was halted. It is not approved by the FDA or any major regulator, and the legal status page covers its compounding and sport position.
What the evidence on AOD-9604 actually shows
Knowing the concentration is not the same as knowing whether to use it. These are the strongest studies behind the AOD-9604 profile, labelled with the study type PubMed itself assigns.
- Observational study · 2001Increase of fat oxidation and weight loss in obese mice caused by chronic treatment with human growth hormone or a modified C-terminal fragmentInternational journal of obesity and related metabolic disorders : journal of the International Association for the Study of Obesity · PMID 11673763
- Journal article · 2014Identification and characterization of peptide drugs in unknown pharmaceutical preparations seized by the Belgian authorities: case report on AOD9604Drug testing and analysis · PMID 24976118
This page is arithmetic on numbers you enter. It does not recommend an amount of AOD-9604, and nothing here is medical advice. For what the published evidence actually supports, read the AOD-9604 profile, and for regulatory status see is AOD-9604 legal. Reviewed June 1, 2026.
Different question? The general reconstitution calculator covers every compound on the site, the blend calculator handles multi-peptide stacks, and vial longevity turns a vial into weeks of supply.
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